Optimization of Enzymatic Hydrolysis Conditions for Production of ACE Inhibitory Peptides

Abstract:

ACE inhibitory peptides are prepared by enzymatic hydrolysis of protein of marine red algae Gracialriopsis lemaneiformis. The hydrolytic activities of four kinds of enzymes of flavourzyme，alkaline protease，trypsin and papain are investigated，and the inhibitory activity of their enzymatic hydrolysates on ACE，and optimization of the digestion process are studied in the paper. Trypsin is considered as the suitable enzyme to prepare ACE inhibitory peptides. Response surface experiments are used to optimize the enzymolysis technology of trypsin based on the results of single factor experiments. Results show that the optimum condition is pH at 8.0，enzyme dosage at 6%，reaction temperature at 35 ℃. Peptides （2.0 mg/mL） prepared under the optimal condition shows a ACE inhibitory activity of 72.37%.