Effects of Cyclocarya paliurus（Batal.） Iljinskaja Extracts on Glucose Consumption of Insulin Resistance HepG2 Cell and Activity of

Abstract:

The effects of Cyclocarya paliurus（Batal.） Iljinskaja Extracts on glucose consumption of insulin resistance HepG2 cell and activity of alpha-glucosidase is studied. HepG2 cell with high concentration insulin（10 μg/mL） is induced to establish insulin resistance cell model and divided model cell into Cyclocarya paliurus polysaccharide（CPP），Cyclocarya paliurus flavone（CPF） and dimethylbiguanide groups. After 24 h administration，glucose assay is used fit to measure the gross glucose consumption of cells（ΔGC）， glucose consumption of unite cell（ΔGC/OD） is detected by MTT assay. The inhibitory activity on alpha-glucosidase of CPP and CPF is evaluated，compared with acarbose，in vitro. Compared with model group，ΔGC and ?驻GC/OD of IR-HepG2 cell is increased by CPF and CPP significantly. CPP and CPF have inhibitory effect on the activity of alpha-glucosidase. Those results indicat that CPF and CPP have effects on increasing the glucose consumption of cell and decreasing the activity of alpha-glucosidase，thus alleviating hyperglycemia.