Optimization of the Fermentation Condition for 2-Keto-D-Gluconic Acid Producing Strain

Abstract:

In order to achieve the high production of 2-keto-D-gluconic acid（2-KGA） by Serratia sp. FMME043，feeding strategy and fermentation conditions are optimized in 30 L fermentor，and fed batch fermentation is established. The optimal fermentation conditions are as follows：the dissolved oxygen is 40%，the quality of 64，80 g，and 96 g ammonium sulfate is added at 16，22 h，and 28 h，respectively，and the quality of 801 g glucose is supplemented with five times at the residual sugar concentration between 15~25 g/L. Under these conditions，at 44 h，the production and the yield of 2-KGA reached 268.5 g/L and 1.04 g/g，which are 58.4% and 9.9% higher than that not optimized，respectively.