Optimization of the Process Conditions for the Degradation of the Lignin in the Cut Stem By Laccase

Abstract:

The experiment uses three factors five levels of orthogonal experimental method to study the different digestion times，enzymolysis temperatures and dosage of laccase influence on cut stem in lignin degradation rate. the change of chemical composition and filling values in cut stem before and after processing also have been measured. The result showed that：（1） Digestion time，enzyme solution temperature，dosage of laccase in cut stem have significantly effected on the degradation of lignin. The best parameter of the enzymatic hydrolysis is time 4 h，enzymolysis temperature 50 ℃，the dosage of laccase 10.0 ?滋L/g. Under this parameter，the lignin degradation effect is best and the degradation rate is as high as 47.3%.（2） The chemical composition of cut stem don't change after the treatment of laccase except lignin and the same situation appeared on the filling valuses. Under the condition of optimal parameters，the use of laccase can effectively degrade lignin，raising the quality and availability of cut stem，and don't lower the quality of cut stem.