Purification and Structure Elucidation of Bioactive Polysaccharide from the Fruiting Bodies of Ganoderma sinense

Abstract:

Crude polysaccharide GS30 precipitated from hot water extracts of the fruiting bodies of Ganoderma sinense were separated by DEAE-column chromatography，and then treated with ethanol precipitation to get a purified polysaccharide fraction GS30S1-20E. Modern analytical techniques，such as IR，NMR，GC/MS and HPSEC were conducted to analyze the structural characteristics of GS30S1-20E. Results showed that GS30S1-20E was a β-D-glucan with a high weight average molecular mass of 2.418×106 and number average molecular weight of 1.668×106，polydispersity of 1.450 and large viscosity of 927.49 mL/g. GS30S1-20E contains a backbone of（1→3）-linked-β-D-glucopyranosyl residues，bearing branches at the O-6 position of every three sugar residues along the backbone. An in vitro immunomodulating activity assay revealed that GS30S1-20E could significantly increase the release of NO from RAW 264.7 cells.