Proteomics of Muscle Protein Changes of Litopenaeus vannamei During Iced Storage

Abstract:

Litopenaeus vannamei is the main shrimp species in our country and popular among consumers. But the shrimp is prone to rapid postmortem quality loss during transportation and circulation，which will reduce its market price. In order to reveal the change rule of Litopenaeus vannamei' muscle protein and screen out the protein indicator of freshness，proteomics technologies were used to study the changes in muscle protein during 0，2，4，6，8 days of postmortem storage. Results shown that differentially expressed protein spots I19 and I14 were generation of degradation products of myosin heavy chain type 1 and adenosine monophosphate deaminase，whose gray value were both on the rise over the storage time. The gray value of myosin heavy chain type 1 shown a good linear positive correlation with iced storage time and its correlation coefficient R2 of 0.920. The gray value of adenosine monophosphate deaminase shown a good quadratic polynomial regression and its correlation coefficient R2 of 0.986. Therefore，myosin heavy chain type 1 and adenosine monophosphate deaminase could indicate the freshness of Litopenaeus vannamei and they could be protein indicators of freshness.