Optimization of Microwave-Ultrasonic Assisted Extraction of Polysaccharides from Hericium erinaceus Antioxidant Activity in vitro

Abstract:

A series of single-factor experiments were made to investigate the effects of particle size，liquid-to-solid ratio，extraction temperature，extraction time，microwave power，ultrasonic power. On the basis of single factor investigation，response surface methodology（RSM） based on Box-Behnken experimental design was used to explore the effect of liquid-to-solid ratio，extraction temperature，extraction time and ultrasonic power on extraction efficiency.The optimum process parameters for an integrated microwave and ultrasonic reaction system extraction process were obtained as：particle size 20，the ratio of water to material 20 mL/g，extraction temperature 74 ℃，extraction time 16 min，microwave power 200 W，ultrasonic power 1 052 W，6.44％ of the yield of polysaccharide was achieved. The predicted values for optimization process conditions were in good agreement with experimental data，indicating that the optimum process parameters by RMS were reliable. The Hericium erinaceus polysaccharides showed potential scavenging activity against hydroxyl hydroxyl，superoxide anionradical and DPPH. This study indicates that Hericium erinaceus polysaccharides are a good source of natural antioxidants.