Study on the Inhibition Against α-glycosidase and Mechanism of Zanthoxylum armature DC.

Abstract:

Study on the inhibition of alkaloids from Zanthoxylum armatum DC. on α-glucosidase and mechanism. The inhibitory activity of alkaloids from Zanthoxylum armatum against α-glucosidase（from yeast and murine small intestine） were evaluated using vitro model，and the mechanism was analyzed using Lineweaver-Burk double-reciprocal analysis. Our results revealed that fat-soluble and dissoluble alkaloids both had significant inhibition on α-glucosidase，IC50 values for α-glucosidase from yeast were （0.73±0.17） mg/mL、（2.74±0.28） mg/mL，respectively. Ones for α-glucosidase from murine small intestine were （1.94±0.13） mg/mL. Moreover，the results of kinetic studies demonstrated inhibitive mechanism of alkaloids is noncompetition inhibition. The results suggested that alkaloids can effectively inhibit the α- glucosidase，and provide strong rationale for further exploitation and utilization.