Application of Fibrous Bed Reactor in the Breeding of Actinobacillus succinogenes Strain

Abstract:

In order to improve the tolerance of Actinobacillus succinogenes to succinate，a microfibrous bed bioreactor with immobilized Actinobacillus succinogenes is constructed in this thesis. Through adaptive evolution，a stain with enhanced salt-tolerance and productivity was obtained with succinic acid concentration of 38.4±3.14 g/L，which was a 23.8% increase compared to the original strain. Fermentative production of succinic acid with both the original and the evoluted strains were conducted in a 3 L fermentor. A fed-batch strategy was used and after 62 h of fermentation，the concentration，productivity and yield of succinic acid was 86.3 g/L，1.39 g/（L·h） and 78.7%，respectively for the evolved strain. Compared to the original strain，the succinic acid yield and productivity increased by 14.2% and 24.1% for the evolved strain.