Separation and Identification of Yeast from Raw Milk in Tacheng of Xinjiang

Abstract:

To study the distribution of yeast in raw milk in pastoral areas of Tacheng，Xinjiang. Seven samples of raw milk were collected from the areas. Using the traditional culture-dependent method，the isolation yeasts were identified according to the colonial morphology，biological properties and 5.8S rDNA sequence homology analysis. A total of 8 species belonging to 6 genera were identified from 33 strains isolated from the isolates，which were 20 strains of Pichia kudriavzevii（61%）were the dominant strains，2 strains Pichia fermentans（6%）；3 strains Metschnikowia pulcherrima（9%）；2 strains Kluyveromyces lactis（6%）；2 strain Kluyveromyces marxianus（6%）；2 strains Rhodotorula mucilaginosa（6%）；1 strain Hanseniaspora uvarum（3%）；1 strain Kazachstania unispora（3%）. The study showed that the yeast diversity of raw milk in this region was good，and has its unique yeast resources. This provides the basic datas for the study of the microbial resources in this area.