Screening of Anti-Breast Cancer Active Fractions in Inonotus obliquus and Its Component Analysis

Abstract:

To achieve this study，MTT assay is used to measure the anti-breast cancer activity in different extracts from Inonotus obliquus；Change of cellular morphology is observed as the apoptosis detection；Cell scratch test is used to determine the effect of drug on cell migration ；LC-MS/MS is used to analyze the active constituents in drug. MTT shows that the petroleum ether extract fraction has the strongest cell growth inhibitory effect on MDA-MB-231 cells in a dose and time dependent manner（P＜0.05）；the IC50 value for 24 hours is 76.17 μg/mL.Cell morphology observation shows that apoptosis effect of petroleum ether extract fraction is increased in MDA-MB-231 cells in a dose manner. Scratch test shows that the petroleum ether extract fraction has a significant inhibitory effect on cell migration. LC-MS/MS data preliminarily characterizes 14 chemical components，of which 10 components are firstly identified in Inonotus obliquus. Although the other 4 substances have not been identified，the molecular weight and molecular formula have been characterized. The petroleum ether extract fraction is considered as the main anti-breast cancer active fraction of Inonotus obliquus. It can inhibit the growth of MDA-MB-231 cells and induce apoptosis，which provided the scientific basis for further development and utilization of the food and medicinal resources of Inonotus obliquus.