Isolation，Identification and Characterization of Cold-Adapted Bacteria from Mingyong Glacier

Abstract:

We screened and identified the cold-adapted bacteria from Mingyong glacier.The bacterial strains were isolated and purified by the soil suspension coating and spread plate methods. Based on physiological and biochemical properties，homology and phylogenetic analysis of 16S rRNA gene sequences，all isolates were classified. In total 22 bacterial strains were isolated，based on the 16S rRNA gene sequence analysis：MY0504 strain was most closely related to Flavobacterium pectinovorum with 97.6%，MY1413 and MY14015 strains were most closely related to Arthrobacter psychrophenolicus and A. parietes with 98% ，while MY14011 strain was most closely related to Chromobacterium fluviatile with 97%，and other 18 strains of bacteria were identified as Pseudomonas spp. The research is preliminary exploration on the microbiological resources in Mingyong glacier，and provides reference information for further studies in this area.