Screening for Cathepsin B Interacting Proteins by Yeast Two-Hybrid System

Abstract:

Cathepsin B is an enzymatic protein that was closely related with tumor cell invasion and metastasis. The network of Cathepsin B is very important for research and development of new anticancer drug，for example，new Cathepsin B inhibitor. Using yeast two hybrid technique，the proteins that interacting with Cathepsin B were identified. Results：After screening analysis，15 positive clones of putative interactions were obtained and they were identified by sequencing and utilizing of the data online（http：//www.ncbi.nlm.nih.gov）. There were 7 genes including BCL2-associated athanogene 6（BAG6），filamin A，alpha（FLNA）， ras homolog family member G（RHOG）， metallothionein 2A（MT2A）， ZXD family zinc finger C（ZXDC）， DEP domain containing 7（DEPDC7），A kinase（PRKA） interacting protein 1（AKIP1）.