Expression of Glucose Oxidase Gene in Yarrowia lipolytica and Fermentation Optimization

Abstract:

Glucose oxidase （β-D-glucose：oxygen 1-oxidoreductase；EC 1.1.3.4，GOD） has gained considerable commercial importance in the last few years due to its multitude of applications in the chemical，food，pharmaceutical，beverage，biotechnology and other industries. In this study，the fermentation medium of the recombinant Yarrowia lipolytica strain 1-28 that was constructed to secrete GOD was optimized through single-factor and orthogonal tests. The optimal fermentation medium was consisted of 20 g/L glycerol，2.64 g/L yeast extract，2.64 g/L NH4Cl，0.13 g/L MgSO4，0.32 g/L KH2PO4，3.34×10-4 g/L Vitamin B1 at pH 6.0. The yield of GOD corresponded to 11.0 U/mL when cultured in the optimized medium，which was 72% higher than that at initial conditions. When fermented in the 3 L tank at 28 ℃ for 190 h，the enzyme activity of GOD reached 81.6 U/mL at pH 5.0 and glycerol feeding at the concentration of 30 g/L.