Cross Priming Amplification with Nucleic Acid Test Strip Analysis of Pork in Meat Mixtures

Abstract:

The phenomenon of meat adulteration has become the concerned problems of the food industry in China. In this study，the cross priming amplification（CPA） and nucleic acid test strip detection method were used together to rapid detect the component of pork meat in the meat product. Specific primers and probes were designed according to the D-loop gene sequence of the pork mitochondria（mtDNA），then the concentration of primers and reaction condition were optimized to establish the best system eventually. The results showed that the amplification system had good specificity for the pork-derived component as the detection limit of the single pork-derived component reached 1 ng/μL，while detection ratio of the pork meat component was 10%（equivalent to 10 ng/μL）. Nucleic acid test strips could quickly get the amplification products，and the result of test strips was as same as that of gel electrophoresis system. The CPA- nucleic acid test strip method established by this study was a specific and efficient method to detect the component of pork meat，which could provide a new effective approach for the quality control of meat products.