Analysis of Trehalose-6-Phosphate Synthase（TPS ） Gene Family and Identification of Ib TPS7a in Sweet Potato（Ipomoea batatas （L.） Lam.）

Abstract:

In order to study the structure and function of TPS family genes in Ipomoea batatas，7 Ib TPS family genes were identified and their sequences，structures and functional properties were determined by bioinformatic method. These Ib TPS genes were divided into class I and class II subfamilies according to their structure and phylogeny relationship. In this study，the complete IbTPS7a gene cloned from Ipomoea batatas contains an open reading frame（ORF ） of 2 568 bp. Meanwhile，Ib TPS7a was transformed into E. coli BL21（DE3） and Saccharomyces cerevisiae INVSc1，respectively. The results indicated that over-expression of IbTPS7a conferred recombinant strains better growth or survival rate than control strain. Digital gene expression profiling（DGE） proved that the expression level of Ib TPS7a has a difference in different tissues，the highest transcription level of Ib TPS7a was in stem and the lowest level was in fibrous root. The results indicated that IbTPS7a gene family existed in sweet potato and Ib TPS7a was helpful to enhance cell resistance to abiotic stress.