Application of SPE-HPLC for Determination of Phenolic Compounds in Chinese Rice Wines

Abstract:

In order to search for a rapid technique for the isolation of polymeric polyphenols from Chinese rice wine for solving the problem that accurately measured the polyphenols in Chinese rice wine because of the impurity interference and low target content. in this paper，the effects of recoveries for the 9 kinds of polyphenol compounds were evaluated by the condition of mobile phase and SPE cartridge types as well as the composition of eluent and the volume of eluent by a high-performance liquid chromatographic method with ultraviolet detection，in the end，using this method for determination polyphenol content in different sweet type of shaoxing Chinese rice wine for precise measurement. Results show that the recovery were between 87.3%~103.8% and the RSD less than3.2% when the Poly-Sery HLB SPE cartridges were used and methanol as eluent and the elution volume for 3 times on the sample volume . catechin and Syringic acid has the highest content in different sweet type of shaoxing Chinese rice wine，the content of polyphenol in dry type and half dry type is lower than the sweet type and half sweet type Chinese rice wine .This SPE method is simple，rapid，and accurate. It can be satisfactorily applied to the determination of polyphenol compounds in wine drinks.