Immobilization of an Sumo-Heparinase I-His Fusion Enzyme on Ni2+ Chelating Agarose Gel Carrier

Abstract:

In this study，immobilization of Sumo-Hep I-His was investigated using Ni2+ chelating agarose gel as carrier. Under optimal conditions，i.e.，weight ratio of barrier to rude enzyme 30∶1，pH 7.0 and immobilized time 6 h，Sumo-Hep I-His was successfully immobilized with an apparent enzymatic activity of 10.07±0.35 IU/mL carrier. Compared with free enzyme，immobilized Sumo-Hep I-His showed great improvement in thermo stability （the half-live was 8 times of free Hep I at 30 ℃ as well as pH stability. The storage and operation stability of the immobilized enzyme was also significantly improved，as 76% relative enzymatic activity was remained after storing for 60 days at 4 ℃ and 75.8% relative enzymatic activity was remained after 8 reaction cycles. GLK-Gel Ni gel also showed good recycling stability that 88.9% adsorptive capacity was remained after 5 cycles immobilization. Conclusively，immobilized Hep I showed great potential in industrial application.