Rapid Screening of High Efficient Lycopene-Producing Mutant by Atmospheric and Room Temperature Plasma Mutagenesis

Abstract:

In order to improve the level of lycopene biosynthesis，this study use atmospheric and room temperature pressure plasma to mutate Blakeslea trispora negative bacteria for lycopene efficient production. The standard curve of lycopene content analysis and the lethal rate curve were established. By constructing the "saddle shaped" lethal rate curve，the suitable mutation treatment time was determined. After several rounds of screening，a mutant strain ARTP-3 was obtained with the lycopene production of 0.85±0.04 g/L，which was increased by 51.4% than that of the original strain. Furthermore，the subculture experiments showed that the ycopene production of ARTP-3 was much stable. In this study，we have not only obtained a high efficient lycopene producer with high economic value，but also can provide useful reference for the breeding of other industrial microorganisms，especially for of filamentous fungi.