Degradation of Chitosan to Produce Bioactive Oligomers Catalyzed by the Porous CLEAs

Abstract:

In order to overcome the defect of low catalytic efficiency of conventional cross-linked enzyme aggregates（CLEAs） toward macromolecular substrate，porous CLEAs（p-CLEAs） was applied in the degradation of chitosan to produce bioactive chitosan oligomers. The reducing sugar concentration was 85% of that catalyzed by free papain，which is 4 times higher than the conventional CLEAs under the same condition. Molecular weight and polydispersity index displayed the particular properties in the degradation of chitosan catalyzed by p-CLEAs compared with free papain. By controlling the degradation time，67.5%（wt） of the oligomer product（5~10 kD） were obtained through ultrafiltration membrane separation. Staphylococcus aureus and Escherichia coli can be totally suppressed when the concentration of oligomers（5~10 kD） was 0.275mg/mL.