Isolation of Siderophore-Producing Microorginisms and Study on It's Application in Fermentation of Green Tea

Abstract:

Siderophores have broad applications in plant nutrition，induction of plant resistance and human health-care.CAS double-layer plateswere employed in order to select siderophore-producing microorganisms. Eight high-yield siderophore-producing Bacillus bacteria strains were selected from the 69 microorganisms which were isolated from roots of cycad. And their siderophores were mainly phenolatescatecholates. Finally，a strain of high siderophore production was chosen，and it revealed to be Bacillus tequilensis by comparsion of 16s rRNA. Bacillus tequilensis produced the best fermented green tea at 40 ℃ and incolubation volume of 0.5 mL/g after 5 d.