Genomic Sequencing and Analysis of Genes Related to PHBV of Halogranum amylolyticum TNN58

Abstract:

Halogranum amylolyticum TNN58 belong to haloarchaeon. It could produce a type of PHBV containing up to 20 mol% 3HV from glucose. This study uses shot-gun sequencing technology to uncover the genome sequence of H. amylolyticum TNN58. Then use Velvet software stitching get 32 scaffolds. The whole genome is about 4.71 Mb，the sequencing depth was 218X. According to the analysis of genome sequence，the PHA sythaseis composed of PhaE and PhaC subunits and there is no acetaldehyde acid cycle in strain. It is interesting to find the methylaspartate cycle is an anaplerotic acetate assimilation pathway. These results will help to reveal the mechanism of PHBV synthesis and provide genome sequence for the research on H. amylolyticum TNN58.