Preparation by Hydrolysis Method and Functional Properties of Yak Bone Collagen Polypeptides

Abstract:

Yak bone protein was hydrolyzed with protease for the preparation of soluble polypeptides. Alkaline protease was proved to be the optimal activator and the single-factor method was used to analyze the effect of time，pH，substrate concentration，enzyme/substrate ratio and temperature on the degree of hydrolysis and yield of polypeptides. Factors such as substrate concentration，enzyme/substrate ratio，hydrolysis pH and time were screened out for response surface analysis based on polypeptides yield. On the optimal hydrolysis conditions of substrate concentration 3.5 g/dL ，enzyme/substrate ratio 4 000 U/g ，hydrolysis pH 7.0 and time 4 h，the actual value of yield was 22.06% and the peptides were detected with good solubility and stability.