Rice Lipase Displayed on Pichia pastoris Surface Catalyzed Synthesis of Ethyl Acetate

Abstract:

In this study，the rice lipase（RL） was displayed on the surfaceof Pichia pastoris to catalyze synthesis of ethyl acetate in n-hexane，the influence of some factors such as the enzyme activity of rice lipase，substrate concentration，reaction temperature，reaction time，the amount of 3A molecular sieve on the synthesis of ethyl acetate was investigated. The study showed that，acetic acid / ethanol（mol/mol） ratio1.2∶1，reaction time of 4 h，reaction temperature of 40 ℃，the enzyme activityof rice lipase 15 750 U/g，the adding amount of 3A molecular sieve8 % in 15 mL n-hexane was the optimum condition . Under the condition，the reaction conversion of rice lipase catalyzed synthesis of ethyl acetate reached 90.92%.