Purification，Structural Characterigation and Immunological Activity of Polysaccharides from the Spores of Ganoderma lucidum

Abstract:

The crude polysaccharides was obtained from the hot-water extract of the spores of Ganoderma lucidum，and two main fractions GLP1a and GLP1b were isolated by DEAE Sepharose Fast Flow chromatography and Sepharose CL-6B gel chromatography. The HPSEC analysis indicated that the two fractions were all homogeneous polysaccharides and their velative molecular mass weight were respectively 1.12×106 and 1.21×105. The analysis of composition，infrared spectrum and NMR spectroscopy indicated that GLP1a and GLP1b all had a backbone of β-D-（1，6） -glucan and GLP1b were partly substituted at C-3 and C-4 in the main chain. Preliminary tests in vitro showed that the two polysaccharide fractions all has stimulating effects on murine splenocyte proliferation and the effect of GLP1b was better than GLP1a. Moreover，their activities were all in a dose-dependent manner. GLP1a.