Screening and Evaluating of Antioxidant Lactobacillus from Gut Microbacterial of Gaotian Villager

Abstract:

To isolate lactic acid bacteria associated with longevity and understand the mechanism about longevity，the gut microbial community of long living elderly residents of Gaotian village was analyzed systematically using 16S high-throughput sequencing. Sequences were performed by species annotation and analyzed the mutual species of gut microbacterial of villagers. This study use MRS to isolated lactobacillus and evaluate the ability of tolerance in simulated gastric and intestinal fluid as well as the ability of scavenging in free radical. Results showed there were 345 OTUs shared all ages of villages in Gaotian. Lactobacillus fermentum 1014 had a high ability of tolerance in simulated gastric and intestinal fluid，best antioxidant ability of scavenging free radical. Those results might help to reveal the phenomenon of longevity and L. fermentum 1014 might be considered as a potential probiotic lactic acid bacterium used as a microbial agent.