Study of the Interaction Between Water-Soluble CdTe Quantum Dots（QDs） and Microcystin-LR Antibody-QDs

Abstract:

Water-soluble CdTe quantum dots（QDs） were synthesized using thioglycollic acid as a stabilizer，and the purified QDs were bind to the Microcystin-LR antibody. The interaction between the QDs and the Microcystin-LR antibody at different temperatures were investigated. The association constant Kapp（84.3M-1），the Stern-Volmer quenching constant Ksv（6.74×104（298 K），6.11×104（304 K），5.93×104（310 K）），the number of binding sites n，and the binding constant K were all calculated through the analysis of UV-Vis absorption and fluorescence spectrum. In addition，the interaction of the thermodynamic parameters between the QDs and the antibody were studied ，△H（＜0），△G（＜0）and △S（＞0），indicating that electrostatic played mainly roles in the interactions between CdTe QDs and Microcystin-LR antibody，and the hydrogen bond and van der Waals also made the contributions.