Effects of Ozone on Zearalenone Degradation and Cytotoxicity Assessment of Ozonolytic Products

Abstract:

An ozonolytic study of zearalenone in acetonitrile solution was conducted under ozone treatment，producing four intermediate compound（m/z for Compound 1=335.184 1，Compound 2 = 351.190 7，Compound 3=321.186 8 and Compound 4=367.175 3）. By means of CCK-8 kit，cytotoxicity assessment of the degraded zearalenone products in HepG2 cells indicates continued toxicity，albeit at decreased levels.AUPLC-MS/MS method has been established to detect the two main products of zearalenoneozonolysis. Both products occur in practical process model for detoxifying contaminated corn flour by ozonation and zearalenone was decomposed by 95.1% in 90 minutes.