Application of Micro Reactor in Pipecolic Acid Synthesis through Enzymatic Catalysis

Abstract:

Pipecolic acid is an important precμrsor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate，a PDMS microchip was designed and fabricated. Effects of microchannel size，microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were：microchannel size 200 μm；microchannel length 124.8 cm；and liquid flow rate 100 μm/min. Under the optimized conditions，a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction，the reaction time reduced 1/3 in comparison with the reaction in shaking tube.