Hydrolysis Characteristics of Candida rugosa Lipase under Ultra High Pressure System

Abstract:

The effects of pressure，temperature，pH and holding time on Candida rugosalipase activity were studied and the recovery of the enzyme was analyzed. We also investigated the changes of kinetics parameters of lipase under different temperature and pressure. The results showed that lipase activity was the highest under 200 MPa and almost 1.76 times of atmospheric pressure. Under this pressure，the optimum temperature and pH for lipase were 40 ℃and pH 7.5，respectively；the processing time was 10 min；the change of Km at 40 ℃ is not as obvious as 50 ℃；meanwhile the Vmax，Kcat and Kcat/Km increased significantly with the elevated pressure.