Construction of Recombinant Saccharomyces cerevisiae for Production of 5-Aminolevulinic Acid

Abstract:

5-aminolevulinic acid（ALA），a naturally occurring non-protein amino acid，has been widely applied in the industry of medicine and agriculture. To construct a recombinant Saccharomyces cerevisiae for production of ALA，genes hem1 cloned from S. cerevisiae and hemA cloned from Rhodobacter spheroides 2.4.1 encoded ALA synthase of C4 pathway was overexpressed，respectively. Meanwhile，glutamyl-tRNA reductase（encoded by hemA） and glutamate-1-semialdehyde aminotransferase（encoded by hemL），the key enzymes of C5 pathway from Escherichia coli were also overexpressed. The result demonstrated that overexpression of hem1 was more beneficial to the production of ALA，327.6 mg/L. Moreover，genes hem1，hemA and hemL were co-overexpressed，resulting recombinant strain IN-PY1AL. The production of ALA was 525.8 mg/L with addition of precursor glycine and succinic acid. This realized the biosynthsis of ALA in S. cerevisiae.