Determination of Marbofloxacin Residues in Milk by Microbiological Method

Abstract:

To establish a microbiological method for determination of marbofloxacin residues in milk. Marbofloxacin residues in milk was tested using filter paper method based on Staphylococcus selected as a sensitive bacteria，and then the recoveries were performed on milk samples to verify the feasibility of this method. Results：the data showed that the linear range for mornitoring marbofloxacin was from 0.1 μg/mL to 1.6 μg/mL based on phosphate buffer solution and milk matrix. The minimum limit detections of marbofloxacin in phosphate buffer solution and milk matrix were 0.1 μg/mL. The average recovery rates were in the range of 77.3%~93.3% and variation coefficient were less than 7.26% for three spiked levels of Erie milk samples. The obtained method can be feasible to mornitoring marbofloxacin residues in milk.