Analysis of Biofilm Formation by Pathogenic and No-Pathogenic Vibrio parahaemolyticus at Various Temperatures and Contact Surfaces

Abstract:

In this study，crystal violet staining method was applied to quantify biofilm-forming ability of pathogenic（n=22） and no-pathogenic（n=17） V. parahaemolyticus on different contact materials（polystyrene，glass and stainless steel） with regard to various temperatures（4，10，15，25 and 37 ℃）. The results showed that V. parahaemolyticus adhered better at high temperatures（25 and 37 ℃） than that at low temperatures（4~15 ℃）. Meanwhile，25 ℃ was the optimal temperature for biofilm formation of V. parahaemolyticus. Compared with the other two materials，the level of bacterial biofilm formation was highest on glass. Based on coefficient of variation（CV） analysis，it displayed that the increase in strain variability of biofilm formation caused by temperature was much greater than that caused by material. Further analysis of the results revealed that the pathogenic bacteria can adhered significantly（p<0.05） better than no-pathogenic bacteria. Therefore，the biofilm formation of pathogenic and no-pathogenic bacteria was obvious difference at five temperatures under three contact surfaces. In future，the contamination caused by pathogenic V. parahaemolyticus biofilm can be controlled effectively through changing temperatures or contact materials during food processing.