Study of Adsorption Capacity of L-Glutamic Acid-Modified Magnetic Chitosan Microspheres on BSA

Abstract:

This experiment explored the adsorption capacity of L-glutamic acid-modified magnetic chitosan microspheres（GA- CMNs） for BSA. GA-CMNs was prepared by reverse phase suspension method，the effect of chitosan glutamic acid connection was detected by infrared spectrometer. Microsphere morphology was detected by SEM，and the particle size distribution of microspheres was detected by laser particle size analyzer. The effects of different pH value，NaCl concentration，BSA concentrations，adsorption time were studied on BSA with GA-CMNs，and the adsorption behavior was analyzed by adsorption kinetics and adsorption thermodynamics. The results showed that the optimum adsorption pH was 5，it was a positive correlation between adsorption time and concentration of BSA to adsorbing capacity of BSA，while the ionic strength was the opposite. The maximum amount of equilibrium adsorption was 83.5 mg/g when initial concentration of BSA was 1 mg/mL. This adsorption behavior was in accordance with the second dynamic model and Langmuir isothermal absorption models，3% NaCl was used as eluent，and elution rate was still higher than 90% when adsorption- elution cycles 5 times. GA-CMNs has good adsorption effect on BSA.