Study on Bioconversion of 2-Phenylethanol by Saccharomyces cerevisiae Resting Cell

Abstract:

The bioconversion conditions on the production of 2-phenylethanol with Saccharomyces cerevisiae resting cell were optimized. Incubation time of resting cell，buffer solution and co-substrate of coenzyme NADH regeneration were determined. Based on the results of single factor test and Plackett-Burm design，three factors including concentration of co-substrate ethanol，pH and reaction temperature were picked out as prominent factors affecting bioconversion of 2-phenylthanol. The optimal bioconversion conditions were determined by Box-Benhnken design and response surface analysis，i.e.，ethanol concentration 16.5 g/L，pH 5.1，temperature 26 ℃，The yield of 2-phenylethanol reached 3.49 g/L，increased by 49.14% compared with the original condition. The cell could maintain their bioconversion activity in repeated utilization at least eight times. After 40 h bioconversion，9.34 g/L of 2-phenylethanol and 83.9% of transformation ratio were obtained under the condition of adding 10% macroporous resin in situ product removal，the yield of 2-phenylethanol increased 171.5%