Establishment of Poria cocos Mycelium Protein 2-DE and MALDI-TOF/TOF Analysis

Abstract:

To establish an efficient 2-DE protocol for analysis of Poria cocos protein and identification of the selected protein spots，the total protein of flask liquid fermented P. cocos was separated by 2-DE and the selected protein spots were analysis by MALDI-TOF/TOF. The results showed an efficient 2-DE of flask liquid fermented P. cocos total protein was established，high quantity protein spots was obtained，most of protein spots had high resolution and clear background，and then the selected protein spots was identified. This study established an useful conduction of 2-DE of P. cocos protein，a high resolution and reproducibility 2-DE image which will be expected to have excellent applications in finding new important functional proteins with proteomics analysis.