Studies on in vitro Activity and Stability of Asparaginase Loaded in Nanospheres

Abstract:

To prepare self-assembly hyaluronic acid-graft-poly（ethylene glycol），（HA-g-PEG） / hydroxypropyl-beta-cyclodextrin（HPCD） hollow nanospheres loaded with AAS（AHHPs），and then investigate the in vitro activity and stability difference between AHHPs and free AAS. AHHPs was prepared and the transmission electron microscopy，size，zeta potential，entrapment efficiency were detected. We investigated the in vitro activity and stability of AHRPs and free AN by measuring the optimum temperature，optimum pH，thermal stability，storage stability，pH stabilities，trypsin stabilities and plasma stabilities. The average particle size was（367.43±2.72） nm，zeta potential was（-15.70±1.25） mV，and the entrapment efficiency of UHPHD was（66.03±3.81） %. The optimum temperature and optimum pH of the AHHPs were 50 ℃ and 7.0，respectively；the optimum temperature and optimum pH of free AAS were 60 ℃and 7.5，respectively. The results of stability experiments showed that the stabilities of AHHPs were significantly superior to the free AAS. AHHPs can improve the activity of AAS and significantly enhance the stabilities of AAS in vitro.