Research on the Eukaryotic Expression of the Non-Cultured Xylanase Gene and the Conditional Optimization of Its Production

Abstract:

The non-cultured microbial xylanase gene X1-19 coming from metagenomics had been expressed on this paper by using Pichia pastoris as recombinant vector. The enzyme production conditions had been optimized. The results were shown as follows：the optimal methanol added final concentration was 1%，inoculum size was 8×108 / mL，the concentration of YNB was 1.2%，the initial pH value was 5，respectively. The highest enzyme yield was （79.0±2.2） U/mL. This research provided the theoretical basis for the heterologous expression of the non-cultured microbial xylanase gene，and had good prospects for industrial application.