Effect of Pleurotus eryngii Different Extracts on Immunity Activity of Mice Macrophages

Abstract:

The present study investigated the immunomodulatory potential of Pleurotus eryngii different polar extracts（PEs） in mice macrophage cell Ana-1，Ana-1 cell proliferative rate were analyzed by MTT assay，cell Ana-1 cytokines secretion（TNF-α and IL-6） were determined by Elisa reaction kit in different extracts concentration and different stimulation time，cell phagocytosis ability was detected by neutral red assay，Toll-like receptors（TLR2 and TLR4） were assayed by Elisa reaction kit. The results showed that，the PEs respectively were shown to be promoter on growth of Ana-1 cell proilferation，in varying degrees. Methanol extracts（PEM） showed the highest promoting Ana-1 cell proliferative activity and cytokines secretion（TNF-α and IL-6）. In concetration of 12.5 μg/mL，the cell proliferative rate and cytokines secretion were reached peak value. After the stimulation by the 12.5 μg/mL PEM，TNF-α reached secretion peak in 12 h and IL-6 reached secretion peak in 24 h，respcetively. Furthermore，PEM could stimulate Ana-1 cell to promote the phagocytosis ability and increase the expressions of TLRs signaling molecules（TLR2 and TLR4）. In conclusion，PEM has significant immunity-stimulating effects by promoting macrophage cell proliferation，inducing cell cytokine secretion and promoting phagocytosis ability.