The Purity of Rice Protein Isolates Subjected

Abstract:

In order to obtain the optimum Impurity removal technologies of rice protein isolates，this paper analysis freeze-thawing pretreatment，enzymatic hydrolysis on，ultrasound，and their synergy on the purity of rice protein isolates. Then the effects of liquid-solid ratio，enzyme concentration，enzymolysis time，enzymolysis temperature and ultrasound power on the purity of rice protein isolates were analyzed. On the basis of single factor experiments，Central Composite design combined with response surface analysis and variance analysis were employed to optimize process parameters. The optimum process conditions were as follows：liquid-solid ratio 6.1 mL/g，enzyme concentration 600 u/g，enzymolysis temperature 62 ℃，enzymolysis time 88min and ultrasound power 140 W. Under these conditions，the theoretical purity of rice protein isolates was 92.68%，which was closely agreed with the experimental value（92.01%）.