Screening and Analyzing of the Hypnotic Active Ingredients of Lilium lancifolium Thunb

Abstract:

To screen and analyze the hypnotic active ingredients of Lilium lancifolium Thunb. Methods：Direct sleep experiment and prolonged pentobarbital sleeping time experiment are used to study the hypnotic active ingredients of Lilium lancifolium Thunb. The hypnotic active ingredients are separated by separation column like macroporous resin column and MCI column. Magnetic resonance imaging（MRI） is used to identify the structure of active ingredients. The n-butanol extract of Lilium lancifolium Thunb can increase the rate and time of sleep，accelerate the sleep of mice. By separating，the main structure of the active ingredients are（25R）-3β，17α-dihydroxy-5α- spirostan-6-one-3-O-α-L-Rhamnopyranosyl-（1→2）-β-D-glucopyranoside and（25R）-3β-hydroxy- 5α-spirostan-6-one-3-O- zα-L-Rhamnopyranosyl-（1→2）-β-D-glucopyranoside. The n-butanol extract of Lilium lancifolium Thunb has significant hypnotic effect and the structure of its main ingredients are saponins.