Relationship between Morphology and Kojic Acid Production by Aspergillus oryzae

Abstract:

To investigate the relationship between morphology and kojic acid production. Methods：Using Aspergillus oryzae to producekojic acid，the effect of critical factors on morphologies was studied by manipulating the concentrationof culture mediumand inoculum level，andfermented by different forms of cellto obtain the relationship between morphology and kojic acid production. Carbon/nitrogen ratio（C/N） of medium has great influence on pellet diameter size of A. oryzae.Dispersed mycelia wereformed with 109 spores mL-1 inoculum，but pellets were obtained under the condition of less spore number107 to 108 spores mL-1，and with the increasing of spores inoculation pellet diameterdecreased. Furthermore，the yield of kojic acid was closely correlated with pellet size，and the optimal pellet diameter range for higher yield of kojic acid on DCW was 0.25 to 0.35 mm.