Separation and Purification of Phycocyanin from Spirulina platensis Based on Ultrafine Cell Breaking Technology

Abstract:

To improve the low efficiency of phycocyanin extracted from Spirulina platensis，the ultrafine shearing method was applied to break the cell of Spirulina，which will provide the basis of industrial extractionof phycocyanin. The single-factor experiments were conducted to study the effects of liquid-solid ratio，ammonium sulfate saturation，time length of ultrafine shearing on theyield of phycocyanin. The response surface methodology was applied to optimize the above processing parameters. The optimum extraction processing parameters based on ultrafine shearing technology were：liquid to solid ratio：30 mL/g，ammonium sulfate saturation：50%，time length of ultrafine shearing：15 min. Under the above optimized conditions，the yield of the phycocyanin was 12%.