Rapid Screening of 28 Banned Veterinary Drugs in Milk by Ultra Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry

Abstract:

An analytical method was established for the simultaneous determination of 28 veterinary drugs in milk by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry（UHPLC-Q-TOF-MS）．The analytes in milk samples were extracted with aeetonitrile containing 0.2％ formic acid．The extract solution was purified by the solid phase extraction cartridge with Oasis PRiME HLB．The target compounds were separated on an Agilent ZORBAX Eclipse Plus C18 column by gradient elution using methanol -0.1％formic acid solution as mobile phase．And detected by MS in ESI+ and ESI- mode．The 28 compounds could be separated in 20 min．As a result，the limits of quantitation（LOQ，S/N=10） of 28 compounds were 1～10 ?滋g/kg in milk．The average recoveries of 28 compounds spiked at three concentration levels in the range of 68.9％～102.0％ with relative standard deviations of 4.30％-11.5％．The accurate mass databases of 28 compounds were established.