Optimization of Preparation and Formula for Beta-Ionone Liposome

Abstract:

To prepare beta-ionone liposomes bythin-film evaporation and ethanol injection using DPPC（1，2-dipalmitoyl-sn-glycero-3-phosphocholine） and HSPC（hydrogenated soy phosphatidy- lcholine） as membrane materials，as well as optimize its preparation formula.The results showed that the optimal preparation condition and formula of beta-ionone liposomes was as follows：the molar ratio of DPPC to HSPCwas 8∶2，the concentration of phosphatidylcholine（PC）was 10 mg/mL，the mass ratio of beta ionone toPCs was 1∶20，the ratio of Tween80 to PCs was 1∶20，the ultrasonic power was 240 W，the time was 4 min.Under these conditions，entrapment efficiency of beta-ionone liposomes was 60.62%±3.48%. The diameters of beta-ionone liposomes were about 200nm，the appearances ofbeta-ionone liposomes were completely or approximately globular vesicles under atomic force microscope（AFM）.