In Vitro Stability of Uricase Self-Assembly Hollow Nanospheres

Abstract:

To prepare the self-assembly hollow nanospheres of hyaluronic acid-graft-poly（ethylene glycol）（HA-g-PEG） and hydroxypropyl-beta-cyclodextrin（HPCD） containing uricase（U HPCD） and study their stability in vitro. UHPCD were prepared and the entrapment efficiency，size and zeta potential of UHPCD were detected. The optimum temperature，optimum pH，thermal stability，storage stability，pH stabilities and proteolytic stabilities of UOX and UHPHD were measured. The entrapment efficiency of UHPHD was（62.17±2.94）%. The average particle size was（299.60±13.05） nm，and the zeta potential was（-45.10±2.75） mV. The optimal temperatures for UHPHD and U0X were 40 ℃，the optimal pH values were 8.5. UHPHD significantly enhanced the stability of free UOX in vitro.