Research of the Pollution of Antibiotic Resistance Genes in Aquatic Products Wholesale Market

Abstract:

In order to investigate the dissemination of antibiotic resistance genes（ARGs） from contaminated aquatic products in aquatic products wholesale market，this study targeted three large-scale aquatic products wholesale markets as research object and collected mixed sewage ditch sediment in and around the three markets separately as test samples. With the help of PCR，this study detected five categories，（four kinds of sulfonamides，eight kinds of tetracyclines，six kinds of β-lactams，five kinds of chloramphenicol，and four kinds of streptomycin） ，in which there is a total of twenty seven kinds of ARGs. Moreover，PCR-DGGE technique was utilized to analyze the diversity of bacteria community in the sample. The results showed that seventeen kinds of ARGs were detected in aquatic products wholesale markets and they all contained multiple types of tetracyclines，sulfonamides，and streptomycin ARGs，which accounted for the conclusion that there was a great variety of ARGs types in aquatic products wholesale market and these three kinds of ARGs were major ones in aquatic products. Sample testing of sewage ditch sediment near the markets showed that ARGs in surrounding place was slightly lower than ARGs in markets，indicating that the occurrence of ARGs in markets would pollute surrounding environment by sewage discharge. Analysis of the results of DGGE profiles showed that though ARGs distributed in three aquaculture markets shared similarities，the distribution of bacterial types between three markets were great different，displaying that there was no direct relationship between the types of ARGs and bacterial types. This work noted that aquatic products wholesale market has became a important receiver of ARGs through the dissemination of ARGs from contaminated aquatic products，and this receiver would not only pollute surrounding environment by sewage discharge to some extent，particularly，resulting a huge risk to pollute aquatic products again，hence needing more attention.