Utilization of Recombinant Carbohydrate Oxidase from Microdochium nivale for Lactobionic Acid Production

Abstract:

The carbohydrate oxidase from Microdochium nivale（MnCO）can catalyze the oxidation of lactose to produce lactobionic acid. In this study，we used the recombinant MnCO which expressed in Pichia pastoris from the genetically engineered bacteria X33-pPICZαA-MnCO stored in our lab. The production of lactobionic acid was optimized by single factor experiment and orthogonal test. The optimal fermentation conditions for the production of lactobionic acid were as follows：the concentration of lactose solution of 5 mmol/L，the amount of enzyme of 1.5 U/ml，the reaction temperature of 55 ℃，the reaction time of 48 h，the conversion rate of lactose to lactobionic acid could reach to 100% .