Effects of Fulvic Acid on Astaxanthin Accumulation and the Transcript Levels Expression Kinetics of lcy Gene of Haematococcus pluvialis LUGU

Abstract:

To explore the effect of Fulvic acid（FA） on Haematococcus pluvialis，different concentration gradient of Fulvic acid（FA） was added in the culture of Haematococcus pluvialis LUGU. Meanwhile，cells growth，astaxanthin accumulation and the transcript levels expression kinetics of lcy gene was studied during induced time. The results show that the suitable concentration of FA not only promote the algae growth（when dealt with 10 mg L-1 FA concentration，the algae biomass production reach the maximum 80.28 mg L-1d-1，increased by 5.44% than that of the control group），but also greatly improve the astaxanthin content（the astaxanthin content reach 20.82 mg L-1 when dealt with 5 mg L-1 FA concentration，is 86.89% higher than the control group）. RT-PCR analysis shows that the transcriptional levels of lcy induced by 5 mg L-1 and 10 mg L-1 FA is 1.2 and 0.7 times than that of control，respectively. The correlation analysis indicate that，the accumulation of astaxanthin content and the transcriptional levels of lcy gene inducted by FA is positively correlated.