Determination of Nucleosides in Caterpillar Fungi by High Performance Liquid Chromatography

Abstract:

In order to afford a reference for the study of nucleosides and quality control of caterpillar fungi products，a method for simultaneous determination 19 kinds of nucleosides using HPLC was established including CMP，UMP，uracil，GMP，IMP，hypoxanthine，uridine，AMP，thymine，adenine， dAMP，inosine，guanosine，2'-deoxyguanosine，thymidine，adenosine，2'-deoxyadenosine，cordycepin， N6-（2-hydroxyethyl）-adenosine. Chromatographic conditions：X select HSS T3 column（5 ?滋m，4.6 mm×250 mm），acetonitrile and water as mobile phase with gradient elution，flow rate 1 mL/min，column temperature 40 ℃，detection wavelength 254 nm. Methodological research showed this was an accurate and reliable method which RSD of precision ranged 0.11%~2.82%，RSD of stability ranged 0.22%~1.63%，RSD of retention time ranged 0.14%~3.47%，RSD of reproducibility ranged 1.3%~5.2%，and recovery ranged 84.9%~108.3%. The nucleoside content in some caterpillar fungi products were successfully determined using this method.